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KMID : 0385220040140010027
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Korean Journal of Gerontology
2004 Volume.14 No. 1 p.27 ~ p.32
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Growth-related Factors as Biomarkers of Brain aging in Rat
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An Duck-Kyu
Seo Joon-Kyo
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Abstract
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In order to identify biomarkers of brain aging, the change in expression of growth-related genes and activities of MAP kinases were measured and analyzed in various tissues of rat brain during aging. The protein levels of p21WAF1/CIP1, p27KIP1, c-fos, c-jun, p-c-jun, c-myc, p-JNK, and p-ERK were measured by Western blotting in cerebral cortex, cerebellum, medulla, olfactory, and hippocampus of 7, 20, 25, and 29-month old rats. The c-myc protein was not detected in cortex, cerebellum, medulla, and olfactory of 7 month-old rats but increased in 20 and 25 month-old rats, except in hippocampus. The c-fos protein showed an increasing pattern in all brain tissues with aging, even though the increase was not as dramatic as that of the c-myc protein. The c-jun protein increased in cortex and olfactory but not in other brain tissues with aging. The p-c-jun increased in all brain tissue except cortex with aging. The level of p-ERK was increasing with aging in all brain tissues except cerebellum. The p-JNK showed different patterns in brain regions with aging. KIP family cyclin dependent kinase inhibitors, p21WAF1/CIP1 and p27KIP1 increased with age in all rat brain tissues. In conclusion we found that c-myc, c-fos, p-c-jun, p-ERK, p21AF1/CIP1, and p27 KIP1 showed increasing in most of brain tissues with aging and could be used as biomarkers of brain aging.
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KEYWORD
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brain aging, immediately early genes, CDK inhibitors, and MAP kinases, and biomarkers
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